Composite

Part:BBa_K1124201:Design

Designed by: Takahiro Yamada   Group: iGEM13_UT-Tokyo   (2013-09-15)

pLux/tet-mCherry-luxI-tetR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1508
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The construct for Multicellular Analog Clock.This BBa_K1124200 is apart of the full clock which lacks sRNA,aiiA,amilGFP,UV system. 

LuxI and LuxR forms the positive feedback loop of AHL production.
TetR is the suppressor for negative feedback loops.
mCherry is the red reporter protein for visualization of the excitation wave (gclock handh).

You can make the full construct of the Multicellular Analog Clock with BBa_K1124100. The excition wave of gene expression are generated on an agar plate and visualized for naked eyes.


Source

Constructed from the Distribution

References